Item 1.01 Entry into a Material Definitive Agreement.

On February 27, 2009, Gen-Probe Incorporated ("Gen-Probe") entered into a Credit Agreement (as amended, the "Credit Agreement") with Bank of America, N.A (the "Lender"), which provided for a one year senior secured revolving credit facility in an amount of up to $180.0 million that is subject to a borrowing base formula.
On March 23, 2009, Gen-Probe entered into an Amendment to Credit Agreement (the "Amendment") with the Lender, pursuant to which the amount which Gen-Probe may borrow from time to time under the Credit Agreement was increased from $180.0 million to $250.0 million. Loans may be borrowed, repaid and reborrowed during the term of the revolving credit facility, and there is currently $170.0 million of borrowings outstanding under the revolving credit facility.
The foregoing description of the Amendment is only a summary, does not purport to be complete, and is qualified in its entirety by reference to the full text of the Amendment, which is filed as Exhibit 10.1 to this Current Report on Form 8-K and is incorporated herein by reference.
Item 2.03 Creation of a Direct Financial Obligation or an Obligation under an Off-Balance Sheet Arrangement of a Registrant.

The information set forth under Item 1.01, "Entry into a Material Definitive Agreement," is incorporated herein by reference.

Item 9.01 Financial Statements and Exhibits.

(d) Exhibits.
10.1 Amendment to Credit Agreement dated as of March 23, 2009 by and between Gen-Probe Incorporated, as Borrower, and Bank of America, N.A., as Lender.

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